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GE Healthcare DeStreak Rehydration Solution

Highly suitable for both analytical and preparative gels. DeStreak Rehydration Solution improves reproducibility and quality of 2-D gels by preventing streaking.

$72.49 - $155.62

Specifications

Product Type Destreak Reagent
For Use With (Application) Improves reproducibility and quality of 2D Gels by preventing streaking
Includes Optimized concentrations of urea, thiourea, CHAPS and Destreak reagent
pH Range >7
Storage Requirements 2° to 8°C
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

45-000-380

 
ge healthcare
17-6003-18
1mL Each for $72.49

45-000-381

 
ge healthcare
17-6003-19
5 x 3mL Pack of 5 Each for $155.62
Description & Specifications

Specifications

Product Type Destreak Reagent
For Use With (Application) Improves reproducibility and quality of 2D Gels by preventing streaking
Includes Optimized concentrations of urea, thiourea, CHAPS and Destreak reagent
pH Range >7
Storage Requirements 2° to 8°C

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs. DeStreak Rehydration Solution effectively eliminates nonspecific oxidation by maintaining protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.

  • Eliminates extra spots caused by nonspecific oxidation of proteins
  • Stabilizes proteins throughout entire 2-D runs, ensuring simplified, well-resolved protein patterns
  • Suitable for both analytical and preparative gels
  • Contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak Reagent

The solution is ready for use after addition of the appropriate IPG Buffer. DeStreak Reagent is also available separately in a 1mL pack size.