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GE Healthcare Con A Sepharose™ 4B Medium

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

$89.28 - $922.56

Specifications

Binding Capacity 30mg/mL
Ligand Concanavalin A
For Use With (Application) For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes
Ligand Concentration 13mg Con A/mL Drained Medium
Matrix 4% Agarose
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

45-000-068

 
ge healthcare
17044001
100mL Each for $922.56

45-000-069

 
ge healthcare
17-0440-03
5mL Each for $89.28
Description & Specifications

Specifications

Binding Capacity 30mg/mL
Ligand Concanavalin A
For Use With (Application) For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes
Ligand Concentration 13mg Con A/mL Drained Medium
Matrix 4% Agarose
Particle Size 90μm
pH Stability 4 to 9
Storage Requirements 2° to 8°C

Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.

  • Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Group-specific adsorbent for molecules containing sugars
  • Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
  • Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
  • Average particle size: 90μm
  • Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl2, 1mM MgCl2, 1mM MnCl2, and 20% ethanol