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Cytiva Amersham™ ECF™ Substrate for Western Blotting
Used for detection of protein blots with fluorescence scanning equipment. Cytiva Life Sciences™ Amersham™ ECF™ Substrate for Western Blotting uses a fluorescent substrate for alkaline phosphatase-based detection of protein blots.
Supplier: Cytiva RPN5785
Includes: ECF Substrate, 60mL; Dilution buffer
Description
Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wavelength.
- For use with fluorescence scanning equipment
- Sensitivity comparable to chemiluminescence
- Wide dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
- Eliminate need for darkroom and film
Western blotting, dot, and slot blotting
Specifications
| Fluorescence scanning equipment | |
| ECF Substrate, 60mL, Dilution Buffer | |
| Sufficient reagents for detecting 2500cm2 membrane |