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GE Healthcare Amersham™ AlkPhos Direct™ Labeling and Detection System with CDP-Star

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes

Specifications

For Use With (Application) All high-sensitivity applications using DNA, RNA, or oligonucleotide probes
Includes Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star detection reagents
Quantity Each
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Products
Catalog Number Mfr. No. Sufficient For Price Quantity    

45-001-776

 
ge healthcare
RPN3691
5000cm2 membrane CALL N/A N/A
 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Description & Specifications

Specifications

For Use With (Application) All high-sensitivity applications using DNA, RNA, or oligonucleotide probes
Includes Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star detection reagents
Quantity Each

AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star™ or ECF™ detection can be used. DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

  • Hybridization stringency can be controlled by elevating temperatures up to 75°C
  • Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
  • ECF detection for quantitation experiments
  • Rapid protocol saves two to three hours over conventional indirect methods

Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm2 or 5000cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star detection reagents.