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GARP Rat anti-Human, eFluor® 660, Clone: G14D9, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS50988241

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Catalog No. 501124576



Description

Description

Description: The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predomit (GARP, also known as LRRC32 or Garpin). GARP is an approximately 80 kDa glycoprotein that is expressed on the cell surface. Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells. The expression of GARP on the surface of activated Treg cells has been reported to be necessary for their suppressive function, possibly related to its role as a cell surface receptor for LAP/TGF beta. Applications Reported: This G14D9 antibody has been reported for use in flow cytometric analysis. Applications Tested: This G14D9 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.
Specifications

Specifications

GARP
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q14392
D11S833E, GARP, garpin, glycoprotein A repetitions predomit, leucine-rich repeat-containing protein 32
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
G14D9
eFluor® 660
LRRC32
Liquid
LRRC32
IgG2a, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
2615
Documents

For Research Use Only.