missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

FOXP3, FITC, clone: FJK-16s, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS11577382

 View more versions of this product

Catalog No. 501129051



Description

Description

FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

71-5775). FJK-16s has also been reported for use in immunohistochemical staining of frozen tissue sections. Applications Tested: This FJK-16s antibody has been tested by intracellular flow cytometric analysis of mouse splenocytes using the Anti-Mouse Foxp3 FITC Staining Set (cat. 71-5775) and protocol. Please refer to intracellular Staining Protocol on Best Protocol web page - Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.
Specifications

Specifications

Foxp3
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q99JB6
AIID, DIETER, Forkhead box P3, foxp3, IPEX, JM2, PIDX, SCURFIN, XPID
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Bovine, Canine, Feline, Mouse, Porcine, Rat
Flow Cytometry, Immunohistochemistry (Frozen)
FJK-16s
FITC
Foxp3
Liquid
FOXP3
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
100037405, 20371, 317382, 444998, 491876, 506053
Documents

For Research Use Only.