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Foxp3 Rat anti-Mouse, PE, Clone: NRRF-30, eBioscience™

Rat Monoclonal Antibody

$346.55 - $535.05

Specifications

Antigen Foxp3
Clone NRRF-30
Host Species Rat
Gene Alias Forkhead Box P3, Scurfin, JM2, Treg
Species Reactivity Mouse
View More Specs

 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

5010778

 
affymetrix
12477180
25μg Each for $346.55

5010779

 
affymetrix
12477182
100μg Each for $535.05
Description & Specifications

Specifications

Antigen Foxp3
Clone NRRF-30
Host Species Rat
Gene Alias Forkhead Box P3, Scurfin, JM2, Treg
Species Reactivity Mouse
Applications Flow Cytometry (Intracellular Staining)
Regulatory Status RUO
Conjugate PE
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.2mg/mL

The NRRF-30 antibody reacts with mouse Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+/CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+/CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with NRRF-30 antibody has mapped the epitope to amino acids 1-75 of the mouse Foxp3 protein.

Intracellular staining of mouse splenocytes with fluorochrome-conjugated NRRF-30 using the eBioscience Foxp3 Staining Buffers (cat. 00-5523) and corresponding staining protocol reveals approximately 3% of total cells in the C57Bl/6 strain and approximately 5% in the BALB/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+/CD25+ cells and 4% of the CD4+/CD25- cells staining with NRRF-30. Co-staining with FJK-16s (anti-mouse/rat Foxp3 cat. 71-5775), which has been mapped to amino acids 71-125, and NRRF-30 shows 100% correlation, indicating that the same cells are stained with both anti-mouse Foxp3 antibodies.

Please see our FAQ regarding the usage of eBioscience Foxp3 reagents.