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Foxp3 Rat anti-Human, eFluor 450, Clone: PCH101, eBioscience™

Rat Monoclonal Antibody

$259.55 - $462.55

Specifications

Antigen Foxp3
Clone PCH101
Host Species Rat
Gene Alias Forkhead Box P3, Scurfin, JM2, Treg
Species Reactivity Human
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

5016358

 
affymetrix
48477641
25 tests Each for $259.55

5016359

 
affymetrix
48477642
100 tests Each for $462.55
Description & Specifications

Specifications

Antigen Foxp3
Clone PCH101
Host Species Rat
Gene Alias Forkhead Box P3, Scurfin, JM2, Treg
Species Reactivity Human
Applications Flow Cytometry (Intracellular Staining)
Regulatory Status RUO
Conjugate eFluor 450
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 5μL (0.5μg)/test

eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells.

The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25bright population. This is subject to donor variability.

PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259).