Please login to your online account to display your discounted pricing

Foxp3 Mouse, Alexa Fluor 488, Clone: 150D/E4, eBioscience™

Mouse Monoclonal Antibody

$187.05 - $462.55

Specifications

Antigen Foxp3
Clone 150D/E4
Host Species Mouse
Gene Alias Forkhead Box P3, Scurfin, JM2, Treg
Species Reactivity Human, Mouse, Rat
View More Specs

 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

5016752

 
affymetrix
53477441
25 tests Each for $187.05

5016753

 
affymetrix
53477442
100 tests Each for $462.55
Description & Specifications

Specifications

Antigen Foxp3
Clone 150D/E4
Host Species Mouse
Gene Alias Forkhead Box P3, Scurfin, JM2, Treg
Species Reactivity Human, Mouse, Rat
Applications Flow Cytometry (Intracellular Staining)
Regulatory Status RUO
Conjugate Alexa Fluor 488
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 5μL (0.5μg)/test

eBioscience offers a panel of monoclonal antibodies to different epitopes of human/primate and mouse/rat Foxp3 protein, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to Foxp3 available from eBioscience, which have been used significantly in scientific literature, include the anti-human Foxp3 PCH101 (cat. 72-5776) and ebio7979 (cat. 12-7979), and the anti-mouse/rat Foxp3 FJK-16s (cat. 72-5775). The 150D/E4 has been mapped to the splice region in Exon 2 found in human cells. The splicing of this region has not been shown to occur in mouse. Characterization of this antibody to the splice variant has been limited to epitope mapping. It should be noted that other antibodies such as PCH101 and 236A/E7 will recognize both the spliced and full length forms of the Foxp3 protein and stain more intensely than 150D/E4 and FJK-16s in human cells.

The 150D/E4 antibody reacts with mouse/rat/human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of FoxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.