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Fisherbrand™ Polypropylene Microtube Support Racks

Excellent for microbiology work or any work using microtubes

Manufacturer:  Thermo Scientific™ 32134

 View more versions of this product

Catalog No. PI32134



Description

Description

Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.

Highlights:

  • Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization

  • Higher sensitivity — detect targets down to the low-picogram level

  • Longer signal duration — sustained light output for as long as five hours

  • More imaging options — X-ray, CCD or laser-based imagers

  • More affordable — high quality and performance at a lower price than other suppliers« similar substrates

Specifications

Specifications

Pierce™
5 hr
Chemiluminescent and Fluorescent
Upon receipt store at 4°C. Product shipped on ice.
2°: 1:25-200K (5-40ng/mL), 1°: 1:1K (0.05- 1µg/mL)
Chemical Substrate
HRP (Horseradish Peroxidase) Substrate
Low picogram to mid-femtogram
SDS
Documents