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Fisher BioReagents™ SurePrep™ FFPE RNA Purification Kit


Provides a rapid method for the isolation and purification of total RNA from formaldehyde-fixed paraffin-embedded (FFPE) tissue samples

Manufacturer: fisher bioreagents™  BP28164

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Catalog No. BP28164

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Description & Specifications

Formalin is used crosslink the RNA and proteins for fixing tissues during tissue preservation. This process of embedding the tissue samples through formalin modification can also lead to fragmentation of the RNA over time. The SurePrep FFPE RNA Purification Kit provides conditions to partially reverse the formalin modifications in order to maximize the yield of functional RNA.

  • Higher yield of RNA contributes to greater accuracy of results in downstream applications
  • The integrity of the RNA sample is retained through the formalin partial reversing process and purification
  • Rapid method for the isolation and purification of all sizes of total RNA which range from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA) less than 200 nt†
  • Spin column method requires five simple steps to isolate and purify RNA from FFPE tissue samples
  • The kit components include DNase to remove any DNA molecules from the sample
  • Total RNA is isolated from FFPE tissue samples without the use of harmful chemicals such as phenol and chloroform
  • The high yield of purified RNA can be used in many sensitive downstream applications for RNA identification and analysis, including QPCR, QRT-PCR, primer extension, and gene expression array assays