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Fisher BioReagents™ LB Broth, Miller (Pre-Buffered Capsules)


Used as a microbial growth medium for the cultivation of E. coli strains for the preparation of plasmid DNA and recombinant proteins.

Manufacturer: fisher bioreagents™  BP9731-5

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Catalog No. BP97315

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Description & Specifications

Specifications

For Use With (Application) For maintenance and propagation of Escherichia coli in molecular biology procedures
Format Pre-Buffered Capsules
Media Format Casein Peptone 10g/L, Yeast extract 5g/L, Sodium Chloride 10g/L, TRIS/TRIS HCl 1.5g/L
Media Type LB Broth
Packaging Poly pail
Product Type LB broth, Miller
Quantity 1000mL Capsules; 5kg

  • Directions for Media Preparation: Add 1 capsule per 1L of purified water, such as BP2820 (Fisher BioReagents Microbial Cell Culture Grade Water). Heat to completely dissolve capsule. Before use, sterilize by autoclaving for 15 minutes.
  • Product is in capsule form
  • Each capsule contains 25g of broth
  • Capsules are prebuffered and set to pH 7.2 at 20°C using a 10mM solution of tris/tris HCl
  • pH will vary with temperature