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Fisher BioReagents™ LB Broth, Miller (Granulated)


Used as a microbial growth medium for the cultivation of E. coli strains for the preparation of plasmid DNA and recombinant proteins.

Manufacturer: fisher bioreagents™  BP9723-500

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Catalog No. BP9723-500

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Description & Specifications

Specifications

For Use With (Application) For maintenance and propagation of Escherichia coli in molecular biology procedures
Format Granulated powder
Media Format Casein Peptone 10g/L, Yeast extract 5g/L, Sodium Chloride 10g/L
Media Type LB Broth
Packaging Poly bottle
Product Type LB broth, Miller
Quantity 500g

  • Directions for Media Preparation: Add 25g to 1L of purified water, such as BP2820 (Fisher BioReagents Microbial Cell Culture Grade Water) then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Before use, sterilize by autoclaving for 15 minutes.
  • Sodium chloride concentration of Miller formulation is 2X the concentration of the Lennox formulation to allow for optimal salt concentration for a specific strain
  • Format: Granulated powder