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Thermo Scientific™ X-Gluc

Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.

Manufacturer:  Thermo Scientific™ R0851

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Catalog No. FERR0851


 

Specifications

Description

X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Recommended for:

  • Detection of GUS expression in plant cells and tissues (1)
  • Detection of infections caused by E.coli
  • Detection of bacterial contamination in food and water samples

Note:

Preparation of a 0.1M stock solution in dimethylformamide or dimethylsulfoxide is recommended.

Description & Specifications

Specifications

X-Gluc
0.1g
Detection of GUS expression in plant cells and tissues (1) Detection of infections caused by E.coli Detection of bacterial contamination in food and water samples