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Thermo Scientific™ X-Gluc


Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.

Manufacturer: thermo scientific™  R0851

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Catalog No. FERR0851

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Description & Specifications

Specifications

For Use With (Application) Detection of GUS expression in plant cells and tissues (1) Detection of infections caused by E.coli Detection of bacterial contamination in food and water samples
Product Type X-Gluc
Quantity 0.1g

X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Recommended for:

  • Detection of GUS expression in plant cells and tissues (1)
  • Detection of infections caused by E.coli
  • Detection of bacterial contamination in food and water samples

Note:

Preparation of a 0.1M stock solution in dimethylformamide or dimethylsulfoxide is recommended.