Thermo Scientific™ Uracil-DNA Glycosylase (UDG, UNG)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
Manufacturer: Thermo Scientific™ EN0361
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. The enzyme shows no activity on RNA.
- Active in Fermentas buffers for restriction enzymes and thermophilic polymerases
- Source: E. coli K12 cells
- Molecular Weight: 25.6 kDa monomer
- The absence of endo-, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests
- E.coli K12 cells
- 25.6kDa monomer
Definition of Activity Unit:
- One unit of the enzyme catalyzes the release 1 nanomole of uracil from uracil-containing DNA template in 60 min. at 37°C
- 30mM Tris-HCl (pH 7.5), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.05% (v/v) Tween 20 and 50% (v/v) glycerol
10X Reaction Buffer:
- 200mM Tris-HCl (pH 8.2 at 25°C), 10mM EDTA, 100mM NaCl
- Inhibitors: Ugi protein from the Bacillus subtilis phage PBS2, protein p56 from the Bacillus subtilis phage phi29 (7).
- Inactivated by heating at 95°C for 10 min. Enzyme activity is partially restored at temperatures lower than 55°C. Therefore put PCR products on ice after PCR and load directly on a gel
Control of carry-over contamination in PCR (2); Glycosylase mediated single nucleotide polymorphism detection (GMPD) (3); Site-directed mutagenesis (4); As a probe for protein-DNA interaction studies (5); SNP genotyping; Cloning of PCR products (6); Generation of single strand overhangs of PCR products and cDNA
The abasic sites formed in DNA by Uracil-DNA Glycosylase may be subsequently cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.UDG (UNG) is active in the presence or absence of divalent cations.
|E. coli K12 cells|
|Ugi protein from the Bacillus subtilis phage PBS2, protein p56 from the Bacillus subtilis phage phi29 (7).|
|25.6 kDa monomer|