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Thermo Scientific™ T3 RNA Polymerase


Catalyze the 5'→3' synthesis of RNA on either single-stranded or double-stranded DNA with T73 RNA polymerase.

Manufacturer: thermo scientific™  EP0102

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Catalog No. FEREP0102

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Description & Specifications

Specifications

Concentration 20U/µL
Description T3 RNA Polymerase
For Use With (Application) Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Includes 1mL of 5X Transcription Buffer
Quantity 10,000U

Bacteriophage T3 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter and is able to incorporate modified nucleotide.>/p>

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter.

  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA–T3 TAATACGACTCACTATA G GGAGA–T7 ATTTAGGTGACACTATA G AAGNG–SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).