Thermo Scientific™ RNase H
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
Manufacturer: Thermo Scientific™ EN0202
Ribonuclease H (Rnase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
- Does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA
- The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
- E.coli MRE-600 cells
- 18.4kDa monomer
Definition of Activity Unit:
- One unit of the enzyme catalyzes the formation of 1nmol of acid soluble products in 20 min. at 37°C
- Enzyme activity is assayed in the following mixture: 20mM Tris-HCl (pH 7.8), 40mM KCl, 8mM MgCl2, 1mM DTT, 24μM [3H]-poly(A)·poly(dT), 0.03mg/mL BSA, 4% (v/v) glycerol
- The enzyme is supplied in: 25mM HEPES-KOH (pH 8.0), 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.1mg/mL BSA and 50% (v/v) glycerol
10X Reaction Buffer:
- 200mM Tris-HCl (pH 7.8), 400mM KCl, 80mM MgCl2, 10mM DTT
Inhibition and Inactivation:
- Inhibitors: metal chelators, SH-blocking reagents
- Inactivated by heating at 65°C for 10 min.
- Removal of mRNA prior to synthesis of second strand cDNA (1)
- RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
- Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT) (2)
- Site-specific cleavage of RNA (3)
- Studies of in vitro polyadenylation reaction products
|metal chelators, SH-blocking reagents|
|E.coli MRE-600 cells|