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Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
Supplier: Thermo Scientific™ EP0441
Description
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
Source
RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
Specifications
• RevertAid Reverse Transcriptase (200 U/μL) |
|
| 60 min. | |
| 42°C | |
| RevertAid | |
| Dry Ice | |
| First-Strand cDNA | |
| Reverse Transcription | |
| RNA |
| Stand-alone Enzyme | |
| Reverse Transcription | |
| 10,000 Units | |
| Yes | |
| 200 U/μL | |
| Separate components | |
| Up to 13 kb |
For Research Use Only. Not for use in diagnostic procedures.