Please login to your online account to display your discounted pricing

Thermo Scientific™ Maxima™ SYBR™ Green/ROX 2X qPCR Master Mix


Optimize qPCR with SYBR Green chemistry using standard cycling protocols with these ready-to-use qPCR master mixes

Manufacturer: thermo scientific™  K0223

 View more versions of this product

Catalog No. FERK0223

  • / Each
 

Description & Specifications

Specifications

For Use With (Application) Real-time PCR using SYBR Green dye; Real-time RT-PCR using SYBR Green dye.
No. of Reactions 4000 reactions of 25 µL
Quantity 4 x 12.5mL

Thermo Fisher Maxima SYBR Green qPCR Master Mixes are ready-to-use solutions optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers.

Only template and primers need to be added. Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). The SYBR Green intercalating dye allows DNA detection and analysis without using sequence-specific probes. ROX serves as a passive reference dye. The use of Maxima SYBR Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. The master mix can be used with most real-time thermal cyclers, including instruments from Applied Biosystems, Eppendorf, Corbett, Bio-Rad and Roche.

  • Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity—detects low copy number targets
  • Wide linear range—accurate quantification across 9 orders of magnitude
  • Universal—can be used on most real-time thermal cyclers
  • Reproducibility and convenience—ready-to-use 2X master mix
  • ROX—supplemented with ROX passive reference dye

Recommended for:

Real-time PCR using SYBR Green dye; Real-time RT-PCR using SYBR Green dye.