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Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X)
Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.
Supplier: Thermo Scientific™ K0221
Description
The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.
- Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity - detects low copy number targets
- Wide linear range - accurate quantification across 9 orders of magnitude
- Reproducibility and convenience - ready-to-use 2X master mix
Specifications
| Contains: • 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 μL reactions. • 2 x 1.25 mL Nuclease-Free Water Store at -20°C. |
|
| Tube | |
| qPCR | |
| Standard | |
| Maxima | |
| 200 Reactions of 25 μL | |
| 2X | |
| DNA, dsDNA |
| SYBR | |
| High | |
| Taq DNA Polymerase | |
| 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System | |
| Real Time PCR SYBR Master Mix | |
| Gene Expression | |
| 200 reactions | |
| 2 x 1.25 mL |
For Research Use Only. Not for use in diagnostic procedures.