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Thermo Scientific™ Maxima™ SYBR™ Green/Fluorescein 2X qPCR Master Mix


Optimize qPCR with SYBR Green chemistry using standard cycling protocols with these ready-to-use qPCR master mixes

Manufacturer: thermo scientific™  K0242

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Catalog No. FERK0242

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Description & Specifications

Specifications

For Use With (Application) Real-time PCR and RT-qPCR using SYBR Green dye on Bio-Rad iCycler iQ, MyiQ or iQ5 machines.
No. of Reactions 1000 reactions of 25 µl
Quantity 10 x 1.25mL

Thermo Fisher Maxima SYBR Green qPCR Master Mixes are ready-to-use solutions optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers.

The master mix includes Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It contains SYBR Green I dye and is supplemented with fluorescein passive reference dye. Only template and primers need to be added.Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

  • Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity—detects low copy number targets
  • Fluorescein—supplemented with fluorescein passive reference dye for use with Bio-Rad iCycler iQ, MyiQ or iQ5
  • Wide linear range—accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience—ready-to-use 2X master mix

Recommended for:

Real-time PCR and RT-qPCR using SYBR Green dye on Bio-Rad iCycler iQ, MyiQ or iQ5 machines.