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Thermo Scientific™ Maxima™ SYBR™ Green 2X qPCR Master Mix with ROX™ Solution


Optimize qPCR with SYBR Green chemistry using standard cycling protocols with these ready-to-use qPCR master mixes

Manufacturer: thermo scientific™  K0252

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 Disclaimers

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

This product is provided under an agreement between Molecular Probes, Inc. and FERMENTAS and the manufacture, use, sale or import of this product is subject to one or more of U.S. Patents, and corresponding international equivalents, owned by Molecular Probes, Inc. (a wholly-owned subsidiary of Invitrogen Corp. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Molecular Probes, Inc., Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA.

The purchase of this product includes a limited, nontransferable license, under specific claims of one or more U.S. patents owned by the University of Utah Research Foundation and/or Idaho Technology, Inc., to use only the enclosed amount of product according to the specified protocols. No right is conveyed, expressly, by implication, or by estoppel, to use any instrument or system under any claim of such U.S. patent(s), other than for the amount of product contained herein.

Use of this product in a passive reference method is covered by the following U.S. Patent: 5,928,907 (claim numbers 12-24, 27-28) and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product in a passive reference method for the purchaser's own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Use of UDG for PCR carryover decontamination is covered by patents in certain countries and may require a license.

Catalog No. FERK0252

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Description & Specifications

Specifications

No. of Reactions 1000 reactions of 25 µl
Quantity 10 x 1.25mL

Thermo Fisher Maxima SYBR Green qPCR Master Mixes are ready-to-use solutions optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers.

The master mix includes Maxima Hot Start Taq DNA polymerase, SYBR Green I dye and dNTPs in an optimized PCR buffer. ROX Solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima Hot Start Taq DNA polymerase in combination with the optimized buffer ensures PCR specificity and sensitivity. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mix in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

  • Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity—detects low copy number targets
  • ROX Solution provided in a separate tube
  • Wide linear range—accurate quantification across 9 orders of magnitude
  • Universal—can be used on most real-time PCR instruments
  • Reproducibility and convenience—ready-to-use 2X master mix

Recommended for:

Real-time PCR and RT-qPCR using SYBR Green dye on most real-time PCR machines.