Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), With Separate ROX Vial
Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.
Manufacturer: Thermo Scientific™ K0251
The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.
- Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity - detects low copy number targets
- Wide linear range - accurate quantification across 9 orders of magnitude
- Reproducibility and convenience - ready-to-use 2X master mix
|Real time PCR (qPCR), gene expression, siRNA validation, genotyping and pathogen detection|
|Store at -20°C.|
|Bio-Rad CFX384 Touch™, BioRad CFX96™, Cepheid SmartCycler, Corbett Rotor-Gene, Eppendorf MasterCycler RealPlex, MJ Chromo4, MJ Opticon, Roche LightCycler 480|
|2 x 1.25mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer, sufficient for 200 x 25μL reactions; 50μL ROX solution, 50μM; 2 x 1.25mL Nuclease-Free Water|
|2 x 1.25mL|
|Detection Method: SYBR; PCR Method: qPCR|