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Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), With Separate ROX Vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Manufacturer:  Thermo Scientific™ K0251

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Catalog No. FERK0251



For Research Use Only. Not for use in diagnostic procedures.



The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.

  • Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity - detects low copy number targets
  • Wide linear range - accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience - ready-to-use 2X master mix
Description & Specifications


Real time PCR (qPCR), gene expression, siRNA validation, genotyping and pathogen detection
200 Reactions
Store at -20°C.
Bio-Rad CFX384 Touch™, BioRad CFX96™, Cepheid SmartCycler, Corbett Rotor-Gene, Eppendorf MasterCycler RealPlex, MJ Chromo4, MJ Opticon, Roche LightCycler 480
2 x 1.25mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer, sufficient for 200 x 25μL reactions; 50μL ROX solution, 50μM; 2 x 1.25mL Nuclease-Free Water
2 x 1.25mL
Detection Method: SYBR; PCR Method: qPCR