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Thermo Scientific™ Maxima™ Hot Start Green 2X PCR Master Mix


Save time preparing samples with this ready-to-use 2x hot start master mix containing tracking dyes and a density reagent for direct loading on gels after PCR.

Manufacturer: thermo scientific™  K1062

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 Disclaimers

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Catalog No. FERK1062

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Description & Specifications

Specifications

For Use With (Application) High throughput hot start PCR; RT-PCR; Highly specific amplification of complex genomic and cDNA templates; Amplification of low copy DNA targets; Generation of PCR products for TA cl
Product Type Fermentas™ Maxima™ Hot Start
Quantity 10 x 1.25mL
Sufficient For 500 reactions of 50 µl

Maxima Hot Start Green PCR Master Mix (2X) is a ready-to-use solution containing Maxima Hot Start Taq DNA Polymerase, optimized hot start PCR buffer, Mg2+, and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of PCR products on a gel.

The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of Maxima Hot Start Taq DNA Polymerase. It is capable of amplification of up to 3 kb from genomic DNA.

The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of PCR products on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of Maxima Hot Start Taq DNA Polymerase. It is capable of amplification of up to 3kb from genomic DNA. For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless Maxima Hot Start PCR Master Mix (2X) (†K0151).

  • Convenient—Maxima Hot Start Taq DNA Polymerase in a ready-to-use mix
  • Hot start PCR—reaction set-up at room temperature
  • Four minute activation time
  • Direct loading of PCR product on gels
  • Highs specificity and sensitivity of PCR

Recommended for:

High throughput hot start PCR; RT-PCR; Highly specific amplification of complex genomic and cDNA templates; Amplification of low copy DNA targets; Generation of PCR products for TA cloning