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Thermo Scientific™ Lambda DNA/HindIII Marker


Perform sizing and approximate quantification of large double-stranded DNA fragments in agarose gels with this conventional marker composed of 8 sharp bands.

Manufacturer: thermo scientific™  SM0102

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Catalog No. FERSM0102

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Description & Specifications

Specifications

Kit Contents 6X DNA Loading Dye, 10 x 1mL
Quantity 25 × 50μg

Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientitfic restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

The marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction.

Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Fermentas PureExtreme restriction enzyme(s), purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker. Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction.

  • Sizing and approximate quantification of large DNA fragments
  • Sharp bands
  • Supplied with loading dye for sample DNA

Note:

The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min. and then cooling on ice for 3 min.