Thermo Scientific™ KspAI (HpaI)
The KspAI (HpaI) restriction enzyme recognizes GTT^AAC sites and cuts best at 37°C in B buffer (Isoschizomers: HpaI).
Manufacturer: Thermo Scientific™ ER1032
Lambda DNA digested with KspAI (HpaI), 0.7% agarose, 14 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.5'..G T T▵A A C...3'
3'..C A A▵T T G...5'
High glycerol (>5%) concentration, pH>8.0 or a large excess of enzyme may result in star activity.