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Thermo Scientific™ Kpn2I (BspEI)


The Kpn2I (BspEI) restriction enzyme recognizes T^CCGGA sites and cuts best at 55°C in Tango buffer (Isoschizomers: AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI).

Manufacturer: thermo scientific™  ER0531

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 Disclaimers

This product or the use of this product is covered US patent application US20100041739A1 and corresponding counterparts owned by Fermentas. The purchase of this product includes a non-transferable license to use this product for the purchaser's internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license from Fermentas.

Catalog No. FERER0531

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Description & Specifications

Specifications

Product Name Kpn2I (BspEI)
Concentration 10U/µL
Units 500U

Lambda DNA digested with Kpn2I (BspEI), 0.7% agarose, 24 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'..T▵C C G G A...3'

3'..A G G C C▵T...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 55°C

Storage Buffer:

  • Kpn2I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: may overlap —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

  • BshTI, BsaWI, Cfr9I, Cfr10I, Eco88I, NgoMIV, SgrAI

Note:

Incubation at 37°C results in 50% activity.