Please login to your online account to display your discounted pricing

Thermo Scientific™ GeneJET™ Plasmid Maxiprep Kit


Perform large-scale isolation of high-quality plasmid DNA from recombinant E. coli cultures with this kit.

Manufacturer: thermo scientific™  K0492

 View more versions of this product

Catalog No. FERK0492

  • / Each
 

Description & Specifications

Specifications

Concentration 400ng/µl
For Use With (Application) Transfection of robust cell lines, FastDigest®, restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,in vitro transcription.
Includes Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Maxi Purification Columns pre-assembled with collection tubes (50mL) and Collection tubes (50mL).
No. of Reactions 25 Preps
Product Type Thermo Scientific™ GeneJET Plasmid Maxiprep Kit

GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription and transfection of robust cell lines.

  • Efficient: High yields up to 750μg of high quality plasmid DNA from 250mL of bacterial culture

  • High concentration of DNA purified: 400ng/μl

  • Convenient: Spin column format, centrifuge or vacuum protocols

  • Fast: Procedure takes 60min, considerably faster than using gravity flow columns

Includes:

Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Maxi Purification Columns pre-assembled with collection tubes (50mL), Collection tubes (50mL)

Recommended for:

Transfection of robust cell lines, FastDigest®, restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,in vitro transcription.