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Thermo Scientific™ GeneJET™ Gel Extraction and DNA Cleanup Micro Kit


Perform rapid and efficient purification of DNA from PCR and enzymatic reaction mixtures and standard or low-melting point agarose gels with this kit.

Manufacturer: thermo scientific™  K0831

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Catalog No. FERK0831

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Description & Specifications

Specifications

Includes GeneJET DNA purification Micro column, Binding buffer, Extraction buffer, Prewash buffer (conc.), Wash buffer (conc.), Elution buffer and collection tube
Storage Requirements Ambient

The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 20 kb. The recovery rates are 90 to 100% for 100 bp to 3 kb DNA fragment size range. Each GeneJET DNA Purification Column has a total binding capacity of up to 10 µg of DNA, and the entire procedure takes approximately 3.5 minutes for DNA cleanup

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 20 kbp. The recovery rates are 90 to 100% for 100 bp to 3 kbp DNA fragment size range. Each GeneJET DNA Purification Micro Column has a total binding capacity of up to 10μg of DNA, and the entire procedure takes approximately 3.5 minutes for DNA cleanup. The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

  • Advanced column design— easy to open, load and handle
  • Yields concentrated DNA— Elute in 6 to 10μL volume
  • High recovery— 90 to 100% recovery in the 100 to 3000 bp DNA fragment range
  • Fast— 4 minute reaction mixture clean-up and 15 minute agarose gel purification protocols available
  • Efficient removal of reaction contaminants– including enzymes, proteins, primers dimers, dNTPs and salts
  • Flexible— does not require precise gel slice quantification weighting

Includes:

GeneJET DNA purification Micro column, Binding buffer, Extraction buffer, Prewash buffer (conc.), Wash buffer (conc.), Elution buffer and collection tube

Recommended for:

Common downstream applications like sequencing, restriction digestion, PCR, qPCR, labeling, ligation, cloning, In vitro transcription, blotting, In situ hybridization