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Thermo Scientific™ IPTG

Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.

Manufacturer:  Thermo Scientific™ R0392

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Catalog No. FERR0392


 

Specifications

Description

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon . It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.

It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening.

Ready-to-use 100mM IPTG aqueous solution is non-toxic, stable, 0.22μm membrane-filtered, formulated for direct use in blue/white colony screening.

Description & Specifications

Specifications

IPTG, dioxane-free
5g
direct use in blue/white colony screening.