Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.
Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems.
- Non-radioactive labeling of DNA
- Efficient priming of labeling reactions with random decamers
- High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction
|Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations|
|Klenow Fragment, exo-;Decanucleotides in Reaction Buffer;Biotin Labeling Mix;Control Template;Biotin-labeled DNA;Water, nuclease-free;Detailed Protocol|