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Thermo Scientific™ DecaLabel™ Biotin DNA Labeling Kit


Efficiently synthesize biotin-labeled DNA probes with the Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit.

Manufacturer: thermo scientific™  K0651

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Catalog No. FERK0651

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Description & Specifications

Specifications

For Use With (Application) Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations
Includes Klenow Fragment, exo-;Decanucleotides in Reaction Buffer;Biotin Labeling Mix;Control Template;Biotin-labeled DNA;Water, nuclease-free;Detailed Protocol
No. of Reactions 10
Product Type Biotin DecaLabel™ DNA Labeling Kit

Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.

Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems.

The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. Klenow Fragment, exo- is also included in the kit; this genetically engineered enzyme has no detectable exonuclease activity. Therefore, the enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. As a result, DNA fragments of any length can be uniformly labeled. Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or with conventional biotin-avidin or biotin-streptavidin detection systems.

  • Non-radioactive labeling of DNA
  • Efficient priming of labeling reactions with random decamers
  • High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction

Recommended for:

Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations