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Thermo Scientific™ Biotin Chromogenic Detection Kit

Perform chromogenic detection of biotinylated nucleic acid probes with the ThermoScientific™ Biotin Chromogenic Detection Kit.

$136.50 - $267.75

Specifications

For Use With (Application) Southern, Northern, dot/slot blots, plaque or colony screening.
Includes Streptavidin-AP Conjugate;BCIP/NBT Solution;Blocking Reagent;10X Blocking/Washing Buffer;10X Detection Buffer;Detailed Protocol
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Products
Catalog Number Mfr. No. No. of Reactions Price Quantity    

FERK0661

 
thermo scientific™
K0661
10 Each for $136.50

FERK0662

 
thermo scientific™
K0662
30 Each for $267.75
Description & Specifications

Specifications

For Use With (Application) Southern, Northern, dot/slot blots, plaque or colony screening.
Includes Streptavidin-AP Conjugate;BCIP/NBT Solution;Blocking Reagent;10X Blocking/Washing Buffer;10X Detection Buffer;Detailed Protocol

Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including the Biotin DecaLabel DNA Labeling Kit. 

Highlights

Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes are then visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Visualization does not require X-ray film or other specific equipment.

Highlights

  • High sensitivity of detection - 0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe
  • Low background due to optimized washing and blocking procedures
  • Non-radioactive detection visualization does not require X-ray film or other specific equipment
  • Fast and convenient - ready-to-use components