Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including the Biotin DecaLabel DNA Labeling Kit.
Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes are then visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Visualization does not require X-ray film or other specific equipment.
- High sensitivity of detection - 0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe
- Low background due to optimized washing and blocking procedures
- Non-radioactive detection visualization does not require X-ray film or other specific equipment
- Fast and convenient - ready-to-use components
|Southern, Northern, dot/slot blots, plaque or colony screening.|
|Streptavidin-AP Conjugate;BCIP/NBT Solution;Blocking Reagent;10X Blocking/Washing Buffer;10X Detection Buffer;Detailed Protocol|