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Thermo Scientific™ BCIP-T


Enhance blue color development with BCIP-T, a chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT.

Manufacturer: thermo scientific™  R0822

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Catalog No. FERR0822

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Description & Specifications

Specifications

For Use With (Application) Detection of alkaline phosphatase activity in:; Southern blotting Western blotting Northern blotting dot/slot blots plaque and colony screening;
Product Type BCIP-T
Quantity 5g

Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan.

Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan (1)

Recommended for:

Detection of alkaline phosphatase activity in:

  • Southern blotting
  • Western blotting
  • Northern blotting
  • dot/slot blots
  • plaque and colony screening

Note:

Preparation of a 50mg/mL stock solution in dimethylformamide is recommended.