Thermo Scientific™ FastDigest™ EcoRV (Eco32I) Promotion
Cut at GAT^ATC sites with Thermo Scientific™ FastDigest™ EcoRV (Eco32I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: EcoRV).
Manufacturer: Thermo Scientific™ PMFD0304
Lambda DNA digested with EcoRI, 1% agarose, 21 cleavage sites
FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
- 100% activity of all FastDigest enzymes in the universal buffer
- 100% buffer compatibility with downstream applications
- Complete digestion in 5-15 minutes
- Direct loading on gels
- No star activity
- 176 FastDigest enzymes available
|FastDigest™ EcoRV (Eco32I)|
|10X FastDigest Buffer and 10X FastDigest Green Buffer|
|Fast clone analysis; Fast preparation of DNA for cloning; Digestion of PCR products; Fast RFLP genotyping; Digestion of difficult-to-cleave DNA|