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Thermo Scientific™ Lab Vision™ FAK (Focal Adhesion Kinase) Ab-1, Rabbit Polyclonal Antibody


Ensure accurate, reproducible results in western blotting and immunofluorescence experiments.

Manufacturer: thermo scientific™ lab vision™  RB477P1ABX

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Catalog No. RB477P1ABX

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Description & Specifications

Specifications

Antigen FAK (Focal Adhesion Kinase) Ab-1
Applications Immunofluorescence
Applications Western Blotting
Classification Polyclonal
Concentration 1mg/mL
Conjugate Unlabeled
Cross Reactivity Canine
Cross Reactivity Frog
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Rat
Format Concentrated
Formulation Ab without BSA/Azide (Frozen)
Host Species Rabbit
Immunogen Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK
Isotype IgG
Quantity 500µL
Regulatory Status RUO
Research Discipline Signal Transduction
Type Primary

Ensure accurate, reproducible results in western blotting and immunofluorescence experiments with Thermo Scientific™ FAK (Focal Adhesion Kinase) Ab-1, Rabbit Polyclonal Antibody.

FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.

Host Species: Rabbit

Isotype: IgG

Species Reactivity: Human, Mouse, Rat, Dog and Frog. Others not known.

Epitope: aa 853-1052

Immunogen: Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK

Molecular Weight: 125 kDa

Positive Control: U118, SW156, HUVEC or IMR-5 cells

Cellular Localization: Cytoplasmic and focal contacts

Recommended for:

  • Western Blotting
  • Immunofluorescence

Note:

USA: RUO; Int'l: RUO