missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Thermo Scientific™ Lab Vision™ FAK (Focal Adhesion Kinase) Ab-1, Rabbit Polyclonal Antibody

Ensure accurate, reproducible results in western blotting and immunofluorescence experiments.

Manufacturer:  Thermo Scientific™ Lab Vision™ RB477P1ABX

 View more versions of this product

Catalog No. RB477P1ABX


 

Specifications

Description

Ensure accurate, reproducible results in western blotting and immunofluorescence experiments with Thermo Scientific™ FAK (Focal Adhesion Kinase) Ab-1, Rabbit Polyclonal Antibody.

FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.

Host Species: Rabbit

Isotype: IgG

Species Reactivity: Human, Mouse, Rat, Dog and Frog. Others not known.

Epitope: aa 853-1052

Immunogen: Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK

Molecular Weight: 125 kDa

Positive Control: U118, SW156, HUVEC or IMR-5 cells

Cellular Localization: Cytoplasmic and focal contacts

Recommended for:

  • Western Blotting
  • Immunofluorescence

Note:

USA: RUO; Int'l: RUO

Description & Specifications

Specifications

FAK (Focal Adhesion Kinase) Ab-1
Polyclonal
Unlabeled
Concentrated
Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK
500μL
Signal Transduction
Polyclonal
Immunofluorescence,Western Blotting
1mg/mL
Ab without BSA/Azide (Frozen)
Rabbit
IgG
RUO
Primary
Canine,Frog,Human,Murine,Rat