Thermo Scientific™ Lab Vision™ FAK (Focal Adhesion Kinase) Ab-1, Rabbit Polyclonal Antibody
Ensure accurate, reproducible results in western blotting and immunofluorescence experiments.
Manufacturer: Thermo Scientific™ Lab Vision™ RB477P1ABX
Ensure accurate, reproducible results in western blotting and immunofluorescence experiments with Thermo Scientific™ FAK (Focal Adhesion Kinase) Ab-1, Rabbit Polyclonal Antibody.FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.
Host Species: Rabbit
Species Reactivity: Human, Mouse, Rat, Dog and Frog. Others not known.
Epitope: aa 853-1052
Immunogen: Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK
Molecular Weight: 125 kDa
Positive Control: U118, SW156, HUVEC or IMR-5 cells
Cellular Localization: Cytoplasmic and focal contacts
- Western Blotting
USA: RUO; Int'l: RUO
|FAK (Focal Adhesion Kinase) Ab-1|
|Ab without BSA/Azide (Frozen)|
|Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK|