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Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin and Labeling Kits

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Manufacturer:  Thermo Scientific™ PI21331

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Catalog No. PI21331


 

Specifications

Description

Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

Highlights:

  • Cleavable analog of Sulfo-NHS-LC-Biotin
  • Can remove biotin label by cleaving spacer disulfide bond with reducing agents, such as DTT
  • Ideal for affinity purification experiments that require efficient elution from immobilized avidin supports (cleaving disulfide bond releases proteins)
  • Long spacer arm reduces steric hindrance for binding interactions
Description & Specifications

Specifications

Amine
EZ-Link Sulfo-NHS-SS-Biotin
606.69
No
100mg
-20°C protected from moisture
Yes
Sulfo-NHS ester of biotin with an ethyl-1,3-dithioproprionate spacer
No
24.3
Approx. 50 uses to label a total of 1099mg of IgG using typical conditions
Yes
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