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Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin and Labeling Kits

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Manufacturer: thermo scientific™  21328

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Catalog No. PI21328

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Description & Specifications


Storage Requirements -20°C protected from moisture
Water Soluble Yes
Spacer Arm Length 24.3
Description EZ-Link Sulfo-NHS-SS-Biotin, No-Weigh Format
Quantity 8 x 1mg
Membrane Permeable No
Sufficient For 8 uses to label a total of 88mg of IgG using typical conditions
Cleavable Yes
Chemical Reactivity Amine
Molecular Weight 606.69
PEGylated No
Formulation Sulfo-NHS ester of biotin with an ethyl-1,3-dithioproprionate spacer

Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.


  • Cleavable analog of Sulfo-NHS-LC-Biotin
  • Can remove biotin label by cleaving spacer disulfide bond with reducing agents, such as DTT
  • Ideal for affinity purification experiments that require efficient elution from immobilized avidin supports (cleaving disulfide bond releases proteins)
  • Long spacer arm reduces steric hindrance for binding interactions