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Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits


Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Manufacturer: thermo scientific™  21327

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Catalog No. PI21327

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Description & Specifications

Specifications

Chemical Reactivity Amine
Cleavable No
Description EZ-Link Sulfo-NHS-LC-Biotin, No-Weigh Format
Formulation Sulfo-NHS ester of biotin with a 6-aminocaproic acid spacer
Molecular Weight 556.59
Membrane Permeable No
PEGylated No
Spacer Arm Length 22.4
Quantity 8 x 1mg
Sufficient For 8 uses to label a total of 96mg of IgG using typical conditions
Storage Requirements -20°C with desiccant
Water Soluble Yes

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Highlights:

  • Water-soluble analog of NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
  • Negative charge of -SO3 group keeps biotinylation localized at the cell surface
  • Creates an amide bond
U.S. Patents 5,892,628 and 5,872,261.