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Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin and Labeling Kits


Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Manufacturer: thermo scientific™  21217

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Catalog No. PI21217

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Description & Specifications

Specifications

Chemical Reactivity Amine
Cleavable No
Description EZ-Link Sulfo-NHS-Biotin
Formulation Sulfo-NHS ester of biotin
Molecular Weight 443.43
Membrane Permeable No
PEGylated No
Spacer Arm Length 13.5
Quantity 50mg
Sufficient For Approx. 25 uses to label a total of 752mg of IgG using typical conditions
Storage Requirements -20°C with desiccant
Water Soluble Yes

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

Highlights:

  • Shorter spacer arm than Sulfo-NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
  • Negative charge of -SO3 keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
  • Water solubility eliminates the need for harmful organic solvents
U.S. Patents 5,892,628 and 5,872,261.