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Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin and Labeling Kits

Biotinylate protein amines and maximize solubility of proteins with this long (29.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.

Manufacturer: thermo scientific™  21329

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Catalog No. PI21329


Description & Specifications


Chemical Reactivity Amine
Cleavable No
Description EZ-Link NHS-PEG4-Biotin, No-Weigh Format
Formulation NHS ester of biotin with a tetraethyleneglycol spacer
Molecular Weight 588.67
Membrane Permeable No
PEGylated Yes
Spacer Arm Length 29
Quantity 8 x 2mg
Sufficient For 8 uses to label a total of 181mg of IgG using typical conditions
Storage Requirements Store in original bottle at 4°C with desiccant
Water Soluble Yes

Thermo Scientific™ EZ-Link NHS-PEG&subscript_4;-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.


  • Water-soluble polyethylene glycol (PEG) spacer arm increases solubility of labeled protein
  • Prevents aggregation of antibodies better than Sulfo-NHS-LC-Biotin
  • Labels at N-terminus or lysine residues in less than 1 hour
  • Thermo Scientific No-Weigh Packaging (8 x 2mg) saves time - preserves reagent activity and prevents cross-contamination between use