Thermo Scientific™ EZ-Link™ NHS-PEG Solid-Phase Biotinylation Kit - 1mL Column
Biotinylate protein amines with greater control over the amine-labeling reaction and antibody cleanup process using this column-based method of antibody biotinylation.
Manufacturer: Thermo Scientific™ 0021440
Thermo Scientific™ EZ-Link NHS-PEG Solid Phase Biotinylation Kits provide an on-column approach to amine-targeted antibody biotinylation that provides greater control over the labeling reaction and clean-up process.This innovative antibody-labeling system uses nickel-chelated agarose to temporarily immobilize antibody molecules via their histidine-rich Fc regions. Once held in place on the resin, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and byproducts are then washed away before recovering the labeled and purified antibody from the resin using a mild imidazole solution. No gel filtration or dialysis is needed. Four kits are available for small (0.1-1mg) or large (1-10mg) antibody samples using either amine-directed (NHS ester) or sulfhydryl-directed (maleimide) labeling reagents.
Fast labeling and purification - the entire procedure takes only one hour (two hours for sulfhydryl labeling kits)
Easy removal of spent and excess labeling reagent - simply wash away the reaction byproducts - no need for dialysis or gel filtration
No dilution effects - solid-phase method allows initially dilute antibodies to be recovered in a smaller volume after labeling
Optimized protocols - specific protocols for antibody ensure appropriate level of labeling (2-5 biotins per antibody molecule), minimizing possibility of inactivation caused by overlabeling
Sufficient reagents for eight biotin-labeling experiments - Thermo Scientific No-Weigh Packaging ensures that the biotin reagent is fully active for eight separate experiments
Immobilized Nickel Chelate Column 1mL, BupH Phosphate Buffered Saline 1 pack (makes 500mL), No-Weigh NHS-PEG4-Biotin Microtubes 8 x 2mg, 4M Imidazole Stock Solution 5mL
|NHS-PEG Solid-Phase Biotinylation Kit|
|1 to 10 mg|
|Sufficient For: 8 labeling reactions, each with 1 to 10 mg of antibody
• Ni-IDA Agarose Column, 1 mL, 1 column
• PBS pack (makes 500mL), 1 pack
• NHS-PEG4-Biotin, 8 x 2 mg
• Imidazole Stock Solution (4M), 5 mL
Store at 4°C.