Please login to your online account to display your discounted pricing

Thermo Scientific Expression Vectors for Pierce™ qIP Kits

Optimize cloning with this group of vectors, which includes epitope-tagged (HA and c-Myc) and TurboLuc-tagged (Tluc) multiple cloning site mammalian expression vectors.

$203.70 - $207.90

Products
Catalog Number Mfr. No. Description Price Quantity    

PI82017

 
thermo scientific
82017
Each for $207.90

PI82018

 
thermo scientific
82018
Each for $207.90

PI82019

 
thermo scientific
82019
Each for $207.90

PI82020

 
thermo scientific
82020
Each for $207.90

PI82023

 
thermo scientific
82023
Each for $203.70

PI82024

 
thermo scientific
82024
Each for $203.70

PI82025

 
thermo scientific
82025
Each for $207.90

PI82026

 
thermo scientific
82026
Each for $207.90

PI82028

 
thermo scientific
82028
Each for $207.90

PI82029

 
thermo scientific
82029
Each for $207.90

PI82031

 
thermo scientific
82031
Each for $207.90
Description & Specifications

Optimize cloning in the Thermo Scientific ™ Pierce™ quantitative IP (qIP) protein interaction system with this group of Thermo Scientific™ pCMV Vectors, which includes epitope-tagged (HA and c-Myc) and TurboLuc-tagged (Tluc) multiple cloning site mammalian expression vectors.

These Thermo Scientific pCMV Vectors include epitope-tagged (HA or c-Myc) and Tluc-tagged (TurboLuc Luciferase) multiple cloning site mammalian expression vectors for use in Pierce quantitative IP (qIP) assays.

Pierce qIP Assays are designed to measure the interaction of two proteins (X and Y) that are transiently co-expressed in mammalian cells as epitope-tagged and luciferase-tagged fusions, respectively. The specific luciferase required is Thermo Scientific TurboLuc (Tluc) Luciferase, which is especially small and bright. Protein interactions are quantified by measuring Tluc Luciferase activity following immunoprecipitation (IP) of epitope-tagged proteins with anti-epitope agarose or magnetic beads. As such, the assay system requires that the genes for proteins-of-interest X and Y be cloned as fusions with epitope (e.g., HA or c-Myc) and Tluc tags, respectively. Several vectors, all based on a common pCMV-MCS map, are available for this purpose. These optimized MCS vectors include varieties with tags at the N- or C-terminus. Also available are ready-to-use, positive and negative control vectors containing genes for BAD, RFP or Bcl-xL proteins.

Highlights:

  • CMV (Cytomegalovirus) promoter for constitutive expression of proteins in mammalian cells
  • Shared multiple cloning site (MCS) in all vectors provides convenience and versatility for transfer of cDNA gene from one plasmid to another
  • Tluc tagged vectors use the small and bright TurboLuc Luciferase gene, providing high expression in mammalian systems and the sensitivity required for quantitative IP
  • HA epitope tag (YPYDVPDYA) and Myc epitope tag (EQKLISEEDL) varieties available
  • N-terminal and C-terminal tagged vectors offered for each tag, enabling optimization of protein function for protein:protein interaction
  • Kanamycin/neomycin marker for drug selection in both bacterial and mammalian cells
  • Complete cloning and subcloning guide available (Tech Tip #77)

Product Details:

All Pierce qIP Vectors contain the same multiple cloning site (MCS) sequences. Therefore, once a gene of interest is successfully cloned into one qIP vector with the proper reading frame, the gene can be moved to another qIP vector without additional PCR amplification. This feature provides the convenient and flexible option to subclone a gene from an N-terminal tag vector to C-terminal tag vector.

BAD and Bcl-xL proteins interact strongly as a protein interaction pair. Thus, pairing an epitope-tagged BAD vector with a Tluc-tagged Bcl-xL vector forms a positive control for Pierce qIP Assays. Red fluorescent protein (RFP) does not interact with either BAD or Bcl-xL. Therefore, pairing an epitope-tagged RFP vector with Tluc-tagged Bcl-xL vector provides a negative control for Pierce qIP Assays. The prepared epitope-tagged control vectors (BAD, RFP) that are included in the Pierce qIP Kits are cloned into the nominal MCS expression vectors using NotI and BglII restriction sites. As such, these control vectors can be used as expression vectors for cloning or subcloning a gene of interest.