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Gibco™ ExpiSf™ Expression System Starter Kit DFS Item

Gibco™ ExpiSf™ Expression System Starter Kit is a baculovirus-insect cell protein expression system for superior yields and consistent performance run after run using a fast, streamlined workflow.

Manufacturer:  Gibco™ LSA38841

Catalog No. A38841



Description

Description

  • A chemically defined, yeastolate-free, animal origin-free medium for consistent cell growth and protein expression
  • Integrated components that work in concert to deliver three times higher protein yields compared to traditional insect expression platforms
  • Robust production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
  • A fast, simplified workflow that allows you to obtain protein in as little as 6-10 days; half the time of traditional protocols
  • Scalable expression that can be applied to cultures from 24-deep well plates to >3L shake flasks
Specifications

Specifications

Sf9, SFM Adapted
Baculovirus
Polyhedrin
Approved for shipment at Room Temperature or on Wet or Dry Ice
• 1000 mL bottle ExpiSf CD Medium, store in the dark at 2–8°C
• 4 x 1 mL ExpiSf Enhancer, 2–8°C
• 1 mL ExpiFectamine Sf Transfection Reagent, 2–8°C
• 2 x 1 vial ExpiSf9 Cells, store in vapor-phase liquid nitrogen
• 500 mL Opti-MEM, 2–8°C
• 10 µg pFastBac1 vector, 2–8°C
• 4 ng pFastBac1-Gus control vector, 2–8°C
• 5 x 0.1 mL MAX Efficiency DH10Bac Competent Cells, –80°C
• 24 x 125-mL Single-Use Nalgene PETG Erlenmeyer Flasks, store at room temperature
ExpiSf™
Untagged
Restriction Enzyme ⁄ MCS
SDS
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