Thermo Scientific™ Exonuclease I (Exo I)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
Manufacturer: Thermo Scientific™ EN0582
Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.
- Active in PCR buffers
- Primer removal from PCR mixtures:
- prior to PCR product sequencing li>for one-tube "megaprimer" PCR mutagenesis
The enzyme is not suitable for removing 3'-overhangs of dsDNA.
|E.coli cells with a cloned E.coli sbcB gene|
|20% (w/v) PEG 8000 (5)|