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Thermo Scientific™ Evolution™ 300 UV-Vis Spectrophotometer

The Thermo Scientific™ Evolution 300 is ideal for life science, pharmaceutical and QA/QC applications. A wide selection of accessories and a low cost of ownership make it a versatile and affordable addition to the lab.

Specifications

Accuracy ±0.004A at 1A, ±0.004A at 2A, ±0.006A at 3AÅ
Accuracy (Photometric) ±0.004A at 1A, ± 0.004A at 2A, ± 0.006A at 3A
Baseline Flatness ±0.0015A (200-800nm), 2.0nm SBW, smoothed
Beam Geometry Dual-Beam; Quartz Coated
Certifications/Compliance 21 CFR Part 11 and audit-proof IQ/OQ/PQ documentation. ISO 9001:2000
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Products
Catalog Number Mfr. No. Description Price Quantity    

14-386-407

  Accessories
thermo scientific™
10300201
Evolution 300 PC UV-Vis Spectrophotometer CALL N/A N/A
 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Description & Specifications

Specifications

Accuracy ±0.004A at 1A, ±0.004A at 2A, ±0.006A at 3AÅ
Accuracy (Photometric) ±0.004A at 1A, ± 0.004A at 2A, ± 0.006A at 3A
Baseline Flatness ±0.0015A (200-800nm), 2.0nm SBW, smoothed
Beam Geometry Dual-Beam; Quartz Coated
Certifications/Compliance 21 CFR Part 11 and audit-proof IQ/OQ/PQ documentation. ISO 9001:2000
Connections RS-232
Depth (English) 21in.
Detector Type Dual Matched Silicon Photodiodes
Lamp Xenon Flash Lamp
Typical lifetime: >5 years, longer if not using live signal
Warranty period: 3 year source replacement warranty
Optical Design

Modified Ebert

Double beam with sample and reference cuvette/accessory positions

Spectral Bandwidth Variable 0.5; 1.0; 1.5; 2.0; 4.0 nm
Drift <0.0005Abs/hr, 500 nm, 2.0 nm SBW, 2hr warm-up
Height (English) 15in.
Includes VisionSecurity Software, User Guide and USB Cable
Interface Computer Control
Monochromator Modified Ebert
Noise

Photometric:

0A: <0.00018 A

1A: <0.00022 A

2A: <0.00050 A

500 nm, 2.0 nm SBW, RMS

Pharmacopoeia Compliance Testing

Resolution (Toluene in Hexane): Peak/Trough Ratio >2.0

Photometric Accuracy (60mg/L K2Cr2O7): <±0.01 A relative to calibrated sealed standard

Stray Light: <0.13 %T at 198 nm with KCl per EP

220 nm: <0.01%T NaI (behaves identically to KI for USP)

Wavelength Accuracy: ±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm

Wavelength Repeatability: Peak separation of repetitive scanning of Hg line source <0.10 nm

Photometric Accuracy Including Standard Tolerance

1A: ±0.008 A

2A: ±0.010 A

3A: ±0.018 A

Photometric Display ±6 A
Photometric Range >4A
Photometric Readout Absorbance, % Transmittance, % Reflectance, Concentration
Photometric Repeatability 1A: ±0.0025 A
Range (Photometric) >4A
Resolution (Toluene/Hexane) Peak/Trough >2.0 at 0.5nm SBW
Scan Ordinate Modes Absorbance, % Transmittance, % Reflectance, Concentration,
1st-4th Derivative
Stray Light

198 nm: 2.9 A KCl

220 nm: 4.2 A NaI

340 nm: 4.3 A NaNO2

System Requirements At least Windows XP
Wavelength Data Interval 10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm
Wavelength Range 190 to 1100nm
Wavelength Repeatability Standard deviation of 10 measurements <0.05 nm
Wavelength Scan Speed 3800, 2400, 1200, 600, 240, 120, 60, 30, 10, 5, 1 nm/min Intelliscan
Weight (English) 48.4lb.
Width (English) 24in.

The Thermo Scientific™ Evolution 300 is ideal for life science, pharmaceutical and QA/QC applications. A wide selection of accessories and a low cost of ownership make it a versatile and affordable addition to the lab. The powerful Xenon lamp-based platform and variable bandwidth make the Evolution 300 ideal for life science, pharmaceutical and QA/QC applications. A selection of VISION software packages expand instrument capability and Smart Accessories make your analyses easier to perform. The walk-up convenience of the Xenon lamp saves valuable time and increases your lab's productivity.

The powerful Xenon lamp-based platform and variable bandwidth make the Evolution 300 ideal for life science, pharmaceutical and QA/QC applications. A selection of VISION software packages expand instrument capability and Smart Accessories make your analyses easier to perform. The walk-up convenience of the Xenon lamp saves valuable time and increases your lab's productivity.


Xenon Lamp

  • Provides excellent performance over the full wavelength range
  • Ready to use without instrument warmup for fast readings
  • Activates only when taking a measurement; extended lamp life lowers cost of instrument ownership
  • 3-year warranty


Key Features

  • 5 variable spectral bandwidth settings for matching customer assays to instrument
  • Acquisition rate: 50 data points per second
  • Longlife Xenon lamp


Optional Smart Accessories For Any Application

  • Full line available for Life Sciences and QA/QC
  • Install or remove without restarting the instrument
  • Built-in software instantly identifies and configures to accommodate accessory
  • Each accessory has a unique serial number that is recorded for comprehensive event logs and audit trail capabilities
  • Automated performance verification with CVC accessory
  • Mercury lamp accessory is available for testing and defining wavelength accuracy


PC Control

  • Enables data collection and distribution to third party applications for advanced processing and archiving
  • Uses powerful VISION software suite to control instrument
  • Collects, stores, recalls and analyzes data

 

Ordering Information:

All accessories are sold separately. Available software packages include VISIONliteMaterialsCalc (for material analysis), VISIONpro (standard UV-VIS), VISIONlife (Life Science), VISIONsecurity (regulated labs), VISIONlite ColorCalc Basic (XYZ, Lab, CIE), and VISIONlite ColorCalc Advanced (>40DAB/EP color check filters, Pt-Co/Alpha color, Gardner and Iodine color, wine analysis). Other accessories include cell changers, cuvet and test holders, calibration carousels, sippers, fiber optic probes, small volume sampling, and accessories for reflectance and rapid mixing.


Recommended for:

  • General Spectroscopy
  • High-resolution UV-Visible spectrscopy
  • Temperature-based investigations

Life Science

  • Nucleic acid assays
  • Protein concentration assays
  • Thermal denaturation/renaturation
  • Kinetics

QA/QC

  • Quantitative analysis
  • Finished product analysis
  • Measurements in the regulated laboratory

Pharmaceuctical

  • General Research
  • QA/QC
  • Tablet dissolution


Note:

To use the Evolution 300 UV-Vis Spectrophotometer in a regulated or quality control environment, we offer a wide selection of compliance products and services to reduce implementation time and assure system performance.