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Thermo Scientific™ EquiPhi29™ DNA Polymerase DFS Item

EquiPhi29 is a proprietary Phi29 DNA Polymerase mutant significantly improved over Phi29 DNA Polymerase in protein thermostability, reaction speed, product yield and amplification bias.

Manufacturer:  Thermo Scientific™ A39392

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Catalog No. A39392



Description

Description

Thermo Scientific EquiPhi29 is a proprietary Phi29 DNA Polymerase mutant developed through in vitro protein evolution. This enzyme is significantly improved over Phi29 DNA Polymerase in protein thermostability, reaction speed, product yield and amplification bias, while retaining all the benefits of the wild type enzyme, including high processivity (more than 70 kb), strong strand displacement activity, 3'→5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA or RNA. For this reason 3'-modified primers are recommended.

  • Lowest amplification bias offered in the market
  • Extremely high yields of amplified DNA even from minute amounts of template
  • Highly accurate DNA synthesis in shorter time

 

Specifications

Specifications

EquiPhi29 DNA Polymerase
• 5 x 100 μL EquiPhi29 DNA Polymerase (10 U/μL)
• 3 x 1 mL EquiPhi29 DNA Polymerase Reaction Buffer (10X)
• 0.25 mL DTT (100 mM)

Store at -5 to -30°C.

Dry Ice
5000 U, 10 U/μL
EquiPhi29 DNA Polymerase
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