missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

EOMES Mouse anti-Human, Porcine, eFluor® 660, Clone: WD1928, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS50487741

 View more versions of this product

Catalog No. 501128864



Description

Description

Description: This WD1928 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R. Applications Reported: This WD1928 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This WD1928 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer Set (cat 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm).

Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
Specifications

Specifications

Eomes
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
O95936
Eomesodermin homolog; EOMES; TBR2
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Porcine
Flow Cytometry
WD1928
eFluor® 660
EOMES
Liquid
EOMES
IgG1, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
100622125, 8320
Documents

For Research Use Only.