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EMD Millipore Luminata™ Western HRP Chemiluminescence Substrates


Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Manufacturer: emd millipore  WBLUR0500

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Catalog No. WBLUR0500

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Description & Specifications

Specifications

Applications Western blotting, dot/slot blotting
Format HRP
Product Type Luminata Crescendo Western HRP Substrate
Quantity 500mL
Sensitivity ∽1 to 3pg
Storage Requirements 2° to 8°C

Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.

  • Consistent results with less pipetting error

The stabilizers added to Luminata substrates:

  • Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
  • Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
  • Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation

Three different sensitivities for a range of detection needs:

  • Luminata Classico premixed reagent for everyday detection needs
  • Luminata Crescendo premixed reagent for detection in the picogram range
  • Luminata Forte premixed reagent for detection in the femtogram range