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EMD Millipore BlØk™ Noise Canceling Reagents

Minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins

Manufacturer:  EMD Millipore WBAVDP001

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Catalog No. WBAVDP001


 

Specifications

Description

Blocking Buffer; Blok-PO; 500mL; For phosphoprotein detection via chemiluminescence or fluorescence; Ready-to-use; Protein-free; 1 yr shelf life; Compatible w/PVDF and nitrocellulose; Sufficient for (19) 8 x 7cm miniblots; Room temp storage

  • Ready-to-use
  • Protein-free, reducing nonspecific binding, especially when using phosphoantibodies or avidin-biotin systems
  • Specially engineered for the EMD Millipore SNAP i.d.™ detection system, but may be used in traditional Western blotting protocols
  • Suitable for the dilution of primary and secondary antibodies
  • Sufficient for 19 miniblots (each 8 × 7cm blot requires at least 26mL for SNAP i.d.)
  • Compatible with streptavidin antibodies
  • Compatible with PVDF (Immobilon™ P, Immobilon FL) and nitrocellulose

  • BlØk-CH reagent is compatible with Immobilon HRP substrate
  • Choose from three BlØk reagents
    • BlØk-CH reagent for HRP chemiluminescence detection
    • BlØk-FL reagent for fluorescence detection
    • BlØk-PO reagent for phosphoprotein detection via chemiluminescence or fluorescence

    Description & Specifications

    Specifications

    Western blotting, dot/slot blotting, mass spectrometry, Phosphorolation assay
    500mL
    20° to 25°C
    Blok-PO Reagent
    Nineteen 8 x 7cm miniblots